Application of elimination voltammetry with linear scan in bioelectrochemistry
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Proceedings |
| Conference | The role of electrochemistry in sustained development of modern societies, 54 th ISE Meeting |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | Voltammetric methods; elimination voltammetry with linear scan (EVLS); elimination functions; adsorbed species; mercury and solid electrodes; resolution of reduction and oxidation signals in oligo- and poly-nucleotides |
| Description | Elimination Voltammetry with Linear Scan (EVLS) is able to eliminate one or two selected current components from a total linear sweep voltammetric current. This is achieved by using elimination function obtained by the linear combination of total currents measured at different scan rates. In comparison with cyclic voltammetry (CV) or linear sweep voltammetry (LSV), the EVLS provides specific advantages: (a) expanded the available electrode potential range, (b) increased current sensitivity, and (c) improved peak resolution. The last two benefits result from the fact that the elimination of kinetic and charging currents decreases the irreversible current width and increases the peak height. The effect is particularly pronounced in the case where adsorption is involved. For the electroactive substance adsorbed or interacting with the electrode surface, the elimination function gives a well detectable peak-counterpeak signal which can be successfully used to analyze overlapped reduction or oxidation signals. The elimination procedure was applied to the analysis of nucleic acids (NA) and short synthetic homo- and hetero-deoxyoligonucleotides (ODNs) containing adenine (A), cytosine (C) and guanine (G). While only an incompletely developed peak can be seen on CV curves, the elimination function eliminating kinetic and charging currents and conserving a diffusion current gives well developed sharp peak-counterpeak signals. The height and position of these signals were affected by pH, buffer components, the content of the bases, and the time of accumulation. The EVLS results showed that the elimination voltammetric signal of C is separated from the signal of A, and the elimination signals of A and C reflect their sequences in ODNs molecules. Using selected elimination functions the EVLS is able to detect the nature of electrode processes. The EVLS, particularly in combination with adsorptive stripping procedure, is a promising tool for qualitative and quantitative electrochemical studies of oligo- and polynucleotides, including hairpins and nucleic acids. |
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