Cleavage of double stranded plasmid DNA by lanthanide complexes
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Proceedings |
| Conference | 3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | plasmid DNA pBR322; cleavage; lanthanide ions; EDTA and IDA complexes; particles |
| Description | The use of free lanthanide ions and their complexes for plasmid DNA pBR322 and chromosomal DNA was studied. Plasmid pBR322 DNA was treated by lanthanide clorides in HEPES buffer at different temperatures. Heterogenous lanthanide complexes of EDTA immobilized on insoluble methacrylate support and IDA immobilized on styrene support were used as catalysts for plasmid DNA pBR322 cleavage, too. |
| Related projects: |