Cleavage of double stranded plasmid DNA by lanthanide complexes

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Authors

RITTICH Bohuslav ŠPANOVÁ Alena FALK Martin BENEŠ Milan HRUBÝ Martin

Year of publication 2003
Type Article in Proceedings
Conference 3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords plasmid DNA pBR322; cleavage; lanthanide ions; EDTA and IDA complexes; particles
Description The use of free lanthanide ions and their complexes for plasmid DNA pBR322 and chromosomal DNA was studied. Plasmid pBR322 DNA was treated by lanthanide clorides in HEPES buffer at different temperatures. Heterogenous lanthanide complexes of EDTA immobilized on insoluble methacrylate support and IDA immobilized on styrene support were used as catalysts for plasmid DNA pBR322 cleavage, too.
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