Identifikace bakterií druhu Bifidobacterium longum pomocí polymerázové řetězové reakce a restrikční analýzy amplifikované ribozomální DNA (ARDRA)
| Title in English | Identification of Bifidobacterium longum by polymerase chain reaction and amplified ribosomal DNA restriction analysis |
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| Authors | |
| Year of publication | 2003 |
| Type | Article in Proceedings |
| Conference | VII. Pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | Bifidobacterium longum; identification; ARDRA |
| Description | Method ARDR is based on amplification of DNA region coding 16S rRNA. Genus Bifidobacterium specific amplicon of the length of 914 bp is amplified with the use of primers PbiF1 and PBiR2. This genus specific amplicon was used for restriction analysis. This procedure can be used for resolution of species B.infantis, B. longum and B. animalis. Using PCR with species specific primers was identified the species B. longum. |
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