Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.

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Authors	HASOŇ Stanislav DVOŘÁK Jakub JELEN František VETTERL Vladimír
Year of publication	2002
Type	Article in Periodical
Magazine / Source	Talanta
MU Faculty or unit	Faculty of Science
Citation
Field	Biophysics
Keywords	electrochemical impedance spectroscopy; EIS; DNA; bis-intercalator; echinomycin;
Description	The capacitance measurements (dependence of the differential capacitance C of the electrode double layer on potential e, C-E curves, electrochemical impedance spectroscopy (EIS - frequency deoendence of the impedance Z of the electrode double layer) and constant current chronopotentiometry (depndence of dt/dE on potential E at constant current, chronopotentiometric stripping analysis - CPSA) have been used for electrochemical study of echinomycin and its interaction with single-stranded (ss) and double-stranded(ds) DNA at the hanging mercury drop electrode (HMDE). The capacitance measurement showed that echinomycin gives a pseudocapacitance redox peak strongly dependent on the a.c.voltage frequency at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. Similar results were obtained using CPSA masurements. Thus capacitance measurements and CPSA can distinguish with the aid of the bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the mercury electrode surface, which promise to use this technique for detection of dsDNA in hybridisation reaction.
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