Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping

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Authors	TRNKOVÁ Libuše KIZEK René VACEK Jan
Year of publication	2002
Type	Article in Periodical
Magazine / Source	Bioelectrochemistry
MU Faculty or unit	Faculty of Science
Citation
Field	Electrochemistry
Keywords	Metallothionein;Chronopotentiometric stripping analysis;Adsorptive transfer stripping;Peak H;Catalytic hydrogen evolution;Effect of aeration
Description	Constant current chronopotentiometric stripping analysis (CPSA) in combination with adsorptive transfer stripping (AdTS) technique was used to study the rabbit liver metallothionein (MT) on a hanging mercury drop electrode (HMDE). Metallothionein yielded a distinct, sharp chronopotentiometric signal at very negative potentials (about -1.7 V ), also known as the "peak H". The height and potential of this peak were dependent on experimental conditions, such as buffer composition, pH, and the presence oxygen in solutions. The peak H was highest in borate buffer with pH close to the isoelectric point (pI) of MT. The chronopotentiometric results contribute to a deeper understanding of the nature of catalytic hydrogen evolution and demonstrate the usefulness of the peak H in peptide and protein research.
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