Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | Scripta medica |
| MU Faculty or unit | |
| Citation | |
| Field | Neurology, neurosurgery, neurosciences |
| Keywords | Regeneration; Peripheral nerve; Extracellular matrix; Immunohistochemistry |
| Description | The extracellular matrix formed in a knitted prosthesis, which was applied between the stumps of a transected sciatic nerve of the rat, was investigated to find out whether it provided suitable conditions for nerve regeneration. After 6 weeks, the prosthesis contained a nerve outgrowth connecting the nerve stumps. The immunohistochemical staining of the cryostat sections through the middle of the nerve prosthesis revealed that thrombospondin, fibronectin, chondroitin sulfate A and C, and type IV collagen were localised in the connective tissue surrounding the nerve outgrowth, in the perineurium of individual minifascicles, as well as in the endoneurium among regenerated nerve fibres. No immunostaining for vitronectin was observed in cross-sections through the nerve outgrowth. Our results suggest that the environment formed during nerve regrowth within a knitted prosthesis provides conditions supporting the regeneration of nerve fibres. |
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