Ultrafast detection of microsatellite repeat polymorphism in endothelin 1 gene by electrophoresis in short capillaries

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Authors

KLEPÁRNÍK Karel MALÁ Zdenka PŘIBYLA Lubomír BLAŽKOVÁ Michaela VAŠKŮ Anna BOČEK Petr

Year of publication 2000
Type Article in Periodical
Magazine / Source Electrophoresis
MU Faculty or unit

Faculty of Medicine

Citation
Field Analytic chemistry
Keywords Denaturing capillary electrophoresis; microsatellite repeats; endothelin-1
Description The methodology and instrumentation for fast denaturing electrophoresis in short capillaries was developed and exemplified by detection of short tandem repeat (CA/CT) polymorphism in the endothelin-1 gene. The use of denaturing electrophoresis in short capillaries with laser-induced fluorescence detection resulted in a reduction of analysis time by a factor 200 when compared to the conventional slab gel electrophoresis.
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