RAD51 separation of function mutation maintenance but preserves DSB repair

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Authors	SON Mi Young BELÁŇ Ondrej ŠPÍREK Mário CIBULKA Jakub NIKULENKOV Fedor KIM You Young HWANG Sunyoung MYUNG Kyungjae MONTAGNA Cristina KIM Tae Moon KREJČÍ Lumír HASTY Paul
Year of publication	2024
Type	Article in Periodical
Magazine / Source	iScience
MU Faculty or unit	Faculty of Medicine
Citation
web	https://doi.org/10.1016/j.isci.2024.109524
Doi	http://dx.doi.org/10.1016/j.isci.2024.109524
Keywords	Properties of biomolecules; Genetics; Molecular biology; Molecular interaction
Description	Homologous recombination (HR) protects replication forks (RFs) and repairs DNA double-strand breaks (DSBs). Within HR, BRCA2 regulates RAD51 via two interaction regions: the BRC repeats to form filaments on single-stranded DNA and exon 27 (Ex27) to stabilize the filament. Here, we identified a RAD51 S181P mutant that selectively disrupted the RAD51-Ex27 association while maintaining interaction with BRC repeat and proficiently forming filaments capable of DNA binding and strand invasion. Interestingly, RAD51 S181P was defective for RF protection/restart but proficient for DSB repair. Our data suggest that Ex27-mediated stabilization of RAD51 filaments is required for the protection of RFs, while it seems dispensable for the repair of DSBs.
Related projects:	Mechanics and execution of homologous recombination - biophysics to the organism Identification and characterization of proteins involved in metabolism of G-quadruplexes and R-loops and molecular mechanisms of their relationship with replication