Determination of nutrient concentration in liquid culture of cyanobacteria Nostoc sp. by capillary electrophoresis and inductively coupled plasma mass spectrometry

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Authors

MELICHEROVÁ Natália VACULOVIČ Tomáš KOCI Radka TRTILEK Martin LAVICKÁ Jana FORET František

Year of publication 2024
Type Article in Periodical
Magazine / Source Analytical biochemistry
MU Faculty or unit

Faculty of Science

Citation
Web https://www.sciencedirect.com/science/article/pii/S000326972400174X?via%3Dihub
Doi http://dx.doi.org/10.1016/j.ab.2024.115630
Keywords Cyanobacteria; Photobioreactor; Capillary electrophoresis; Nutrient uptake
Description In this work, we demonstrate the use of capillary electrophoresis and inductively coupled plasma mass spectrometry, as competitive methods primarily for ion chromatography, to determine changes in the concentration of small inorganic ions in the Nostoc sp. culture medium. Although macronutrients were analyzed by capillary electrophoresis with conductivity detection, micronutrients were analyzed by inductively coupled plasma mass spectrometry. The different light settings (light intensity and spectral composition) had a visible effect on the culture growth and depletion of calcium, magnesium, and phosphate ions, and iron and manganese elements when comparing the behavior under red or violet light with that under blue light.
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