Laser-induced breakdown spectroscopy in analysis of algae
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| Year of publication | 2024 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
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| Description | Green algae Desmodesmus subspicatus are single cell freshwater organisms capable of heavy metals accumulation from water. They are analyzed with Inductively Coupled Plasma Mass Spectrometry (ICP-MS) but the parent solution with algae is filtered, the filter with an algae sediment is decomposed and the resulting solution is analyzed with ICP-MS. However, such procedure is laborious and time-consuming. An alternative technique is Laser-Induced Breakdown Spectroscopy (LIBS) with a Czerny-Turner spectrometer in a double pulse (DP) 1064-1064nm configuration [1]. It is sufficiently sensitive to the heavy metals concentrations of about tens of ng cm-2 on the filter surface. A semiquantitative analysis is then possible. Besides, some interesting facts about LIBS of the cellulose filter were revealed. Contaminated algae show monotonous decrease of lines intensities with number of used layers of adhesive tapes for fixing the filter to a microscope glass while blank and non-contaminated samples do not. Also the total emissivity, electron number density and the matrix elements lines are less intensive on the blank than on the contaminated sample. Analogical behaviour shows also plasma shockwave from shadowgraphy experiments. Behaviour of the analyte and the matrix lines is more similar if their signal-to-noise ratio is high or they have similar excitation energies which can be important for possible internal standardization. |
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