Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex

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Authors

ŠTEFANOVIE Barbora JENNER Leon Paul BOZDĚCHOVÁ Lucie FAJKUS Petr SÝKOROVÁ Eva FAJKUS Jiří PALEČEK Jan

Year of publication 2024
Type Article in Periodical
Magazine / Source Plant Molecular Biology
MU Faculty or unit

Faculty of Science

Citation
Web https://link.springer.com/article/10.1007/s11103-024-01461-w
Doi http://dx.doi.org/10.1007/s11103-024-01461-w
Keywords A.thaliana telomerase; AtTERT; AtTR; Protein-RNA interactions; Yeast three-hybrid
Attached files
Description Most eukaryotic organisms employ a telomerase complex for the maintenance of chromosome ends. The core of this complex is composed of telomerase reverse transcriptase (TERT) and telomerase RNA (TR) subunits. The TERT reverse transcriptase (RT) domain synthesises telomeric DNA using the TR template sequence. The other TERT domains contribute to this process in different ways. In particular, the TERT RNA-binding domain (TRBD) interacts with specific TR motif(s). Using a yeast 3-hybrid system, we show the critical role of Arabidopsis thaliana (At) TRBD and embryophyta-conserved KRxR motif in the unstructured linker preceding the TRBD domain for binding to the recently identified AtTR subunit. We also show the essential role of the predicted P4 stem and pseudoknot AtTR structures and provide evidence for the binding of AtTRBD to pseudoknot and KRxR motif stabilising interaction with the P4 stem structure. Our results thus provide the first insight into the core part of the plant telomerase complex.
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