Single particle inductively coupled plasma mass spectrometry imaging of immunochemically labeled spheroids

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Authors

SVOJANOVSKÝ Vilém STIBOREK Marek STRÁNÍK Jaromír JINDŘICHOVÁ Lenka KRÁSENSKÝ Pavel NAVRÁTILOVÁ Jarmila ADAMOVÁ Barbora KANICKÝ Viktor PREISLER Jan

Year of publication 2023
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Spheroids are one of the 3D biological models in which the arrangement of proliferating and necrotic cells resembles the tumor microenvironment. One of the markers of proliferation is Ki-67 protein, which is expressed by cells in the active phase of the cell cycle. Immunohistochemical labeling with a primary antibody and a fluorophore-labeled secondary antibody followed by fluorescence microscopy analysis is commonly employed for two- and three-dimensional imaging of the target protein in spheroid sections. We have successfully switched from fluorophore labeling of Ki-67 to nanoparticle labeling combined with LA ICP MS.* Recently, gold nanoparticles were used as very sensitive immunochemical tags in conventional UV laser ablation ICP MSI.1,2 In our approach, intact 20 nm gold nanoparticles tags were desorbed from the spheroid section with 2 940 nm laser ablation system.
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