Nanospray-assisted deposition of silver nanoparticles for mapping of a peptide in nanofibrous layers via surface-enhanced Raman spectrometry

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Authors

JONAS Vladimír TÝČOVÁ Anna PŘIKRYL Jan KOTZIANOVÁ Adéla VELEBNÝ Vladimír FORET František

Year of publication 2023
Type Article in Periodical
Magazine / Source Talanta
MU Faculty or unit

Faculty of Science

Citation
Web https://doi.org/10.1016/j.talanta.2023.124313
Doi http://dx.doi.org/10.1016/j.talanta.2023.124313
Keywords Colloid; Nanofibers; Nanoparticles; Nanospray; Mapping; Surface-enhanced Raman spectrometry
Description Surface-enhanced Raman spectrometry (SERS) is a universal detection tool identifying molecules via vibrations of their chemical bonds. Its function requires the close localization of metal nanostructures and the analyte. In this work, we present a lab-made instrumentation for the deposition of silver nanoparticles on a strongly hydrophilic nanofibrous composite via a nanospray for SERS mapping of an incorporated peptide. The nanospray-sample distance was revealed as the most crucial parameter since it directly influences the moisture of the deposited colloid. Residual water was recognized as a sensitivity enhancer. Additionally, we continuously introduced a solution of sodium chloride to the colloid increasing its ionic strength, which formed a more homogeneous profile of the deposit. After the deposition process, the treated sample was scanned via a SERS laser and the collected Raman spectra were transformed into a distribution map of the peptide at a concentration of 5?µg/g.
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