The Absence of Retroelement Activity Is Characteristic for Childhood Acute Leukemias and Adult Acute Lymphoblastic Leukemia

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Authors

URAZBAKHTIN Shamil SMIRNOVA Anastasia VOLAKHAVA Anastasiya ZERKALENKOVA Elena SALYUTINA Maria DOUBEK Michael JELINKOVA Hana KHUDAINAZAROVA Nelly VOLCHKOV Egor BELYAEVA Laima KOMECH Ekaterina PAVLOVÁ Šárka LEBEDEV Yuri PLEVOVÁ Karla OLSHANSKAYA Yulia KOMKOV Alexander MAMEDOV Ilgar

Year of publication 2022
Type Article in Periodical
Magazine / Source International Journal of Molecular Sciences
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://www.mdpi.com/1422-0067/23/3/1756
Doi http://dx.doi.org/10.3390/ijms23031756
Keywords acute leukemia; retroelements; mobile elements; tumor-specific insertions
Description Retroelements (RE) have been proposed as important players in cancerogenesis. Different cancer types are characterized by a different level of tumor-specific RE insertions. In previous studies, small cohorts of hematological malignancies, such as acute myeloid leukemia, multiple myeloma, and chronic lymphocytic leukemia have been characterized by a low level of RE insertional activity. Acute lymphoblastic leukemia (ALL) in adults and childhood acute leukemias have not been studied in this context. We performed a search for new RE insertions (Alu and L1) in 44 childhood ALL, 14 childhood acute myeloid leukemia, and 14 adult ALL samples using a highly sensitive NGS-based approach. First, we evaluated the method sensitivity revealing the 1% detection threshold for the proportion of cells with specific RE insertion. Following this result, we did not identify new tumor-specific RE insertions in the tested cohort of acute leukemia samples at the established level of sensitivity. Additionally, we analyzed the transcription levels of active L1 copies and found them increased. Thus, the increased transcription of active L1 copies is not sufficient for overt elevation of L1 retrotranspositional activity in leukemia.
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