Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

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Authors	SOURALOVÁ Tereza HOLUBCOVÁ Zuzana KYJOVSKÁ Drahomíra HAMPL Aleš KOUTNÁ Irena
Year of publication	2021
Type	Article in Periodical
Magazine / Source	Stem Cell Research
MU Faculty or unit	Faculty of Medicine
Citation
Web	https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub
Doi	http://dx.doi.org/10.1016/j.scr.2021.102574
Keywords	human embryonic stem cells; Xeno- and feeder-free derivation; two sex-discordant sibling lines
Description	Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.
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