miR-181a-2*expression is different amongst carcinomas from the colorectal serrated route

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.
Authors

KONDELOVÁ Alexandra ALBURQUERQUE-GONZALEZ B. VYCHYTILOVÁ Petra GARCIA-SOLANO J. PROCHAZKA V. KALA Z. PEREZ F. SLABÝ Ondřej CONESA-ZAMORA P.

Year of publication 2020
Type Article in Periodical
Magazine / Source MUTAGENESIS
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://academic.oup.com/mutage/article-abstract/35/3/233/5648152?redirectedFrom=fulltext
Doi http://dx.doi.org/10.1093/mutage/gez039
Keywords CPG ISLAND METHYLATION; MICROSATELLITE INSTABILITY; BRAF MUTATION; CANCER; EXPRESSION; POLYPS; ADENOCARCINOMA; MICRORNA-31; BIOMARKERS; ADENOMAS
Description Serrated adenocarcinoma (SAC) and colorectal carcinomas showing histological and molecular features of high-level of microsatellite instability (hmMSI-H) are both end points of the serrated pathway of colorectal carcinogenesis. Despite common features (right-sided location, CpG island methylation phenotype and BRAF mutation) there are no studies comparing the microRNA (miRNA) expression profiles in SACs and hmMSI-H.The microtranscriptome from 12 SACs and 8 hmMSI-H were analysed using Affymetrix GeneChip miRNA 3.0 arrays and differentially enriched functions involving immune response were observed from this comparison. miR-181a-2* was found significantly more expressed in hmMSI-H than in SAC and higher expression of this miRNA in microsatellite unstable colorectal cancer were corroborated by Real-Time PCR in an extended series (61 SAC, 21 hmMSI-H). An analysis of genes possibly regulated by miR-181a-2* was carried out and, amongst these, an inverse correlation of NAMPT with miR-181a-2* expression was observed, whereas, for TRAF1 and SALL1, additional regulation mechanisms involving CpG island methylation were observed. miR-181a-2* is associated with particular histological and molecular features of colorectal carcinomas within the serrated pathological pathway and might play a role in the immune responses of microsatellite instability carcinomas.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.