Primary Mammary Organoid Model of Lactation and Involution
| Authors | |
|---|---|
| Year of publication | 2020 |
| Type | Article in Periodical |
| Magazine / Source | Frontiers in Cell and Developmental Biology |
| MU Faculty or unit | |
| Citation | |
| web | https://www.frontiersin.org/articles/10.3389/fcell.2020.00068/full |
| Doi | http://dx.doi.org/10.3389/fcell.2020.00068 |
| Keywords | 3D culture; fibroblast growth factor 2; involution; lactation; mammary gland; milk production; organoid; prolactin |
| Description | Mammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy and lactation, and involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium, as well as surrounding stroma. Three-dimensional (3D) mammary organoid culture has become an important tool in mammary gland biology and enabled invaluable discoveries on pubertal mammary branching morphogenesis and breast cancer. However, a suitable 3D organoid model recapitulating key aspects of lactation and involution has been missing. Here, we describe a robust and straightforward mouse mammary organoid system modeling lactation and involution-like process, which can be applied to study mechanisms of physiological mammary gland lactation and involution as well as pregnancy-associated breast cancer. |
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