In-Cell NMR Spectroscopy of Nucleic Acids in Human Cells
| Authors | |
|---|---|
| Year of publication | 2019 |
| Type | Article in Periodical |
| Magazine / Source | Current Protocols in Nucleic Acid Chemistry |
| MU Faculty or unit | |
| Citation | |
| Web | https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpnc.71 |
| Doi | http://dx.doi.org/10.1002/cpnc.71 |
| Keywords | DNA; eukaryotic cellin-cell NMR spectroscopy; mammalian cell |
| Description | In-cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near-to-atomic resolution. In this article, detailed instructions are described for setting up an in-cell NMR experiment for monitoring structures of DNA oligonucleotides introduced into nuclei of living human cells via tailored electroporation. Detailed step-by-step protocols for both the preparation of an in-cell NMR sample as well as protocols for conducting essential control experiments including flow cytometry and confocal microscopy are described. The strengths and limitations of in-cell NMR experiments are discussed. |
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