Generation of human iPSCs from fetal prostate fibroblasts HPrF

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Authors	KAHOUNOVA Zuzana SLABAKOVA Eva BINO Lucia REMSIK Jan FEDR Radek BOUCHAL Jan VRTEL Radek JUREČKOVÁ Lucie POROKH Volodymyr PARALOVA Darja HAMPL Aleš SOUCEK Karel
Year of publication	2019
Type	Article in Periodical
Magazine / Source	Stem cell research
MU Faculty or unit	Faculty of Medicine
Citation
Web	https://www.sciencedirect.com/science/article/pii/S1873506119300352
Doi	http://dx.doi.org/10.1016/j.scr.2019.101405
Keywords	iPSCs; fetal prostate fibroblasts
Description	Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune (TM)-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.
Related projects:	Centrosomální abnormality u lidských pluripotentních kmenových buněk National research infrastructure for biological and medical imaging Extracelulární faktory ovlivňující metabolismus centrozomů v lidských pluripotentních kmenových buňkách