Development of fast and sensitive CE-MS method for determination of drug-plasma protein binding parameters
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| Year of publication | 2018 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Drug binding to plasma proteins in the blood affects both their pharmacodynamics and pharmacokinetics, which include drug liberation, adsorption, disposition, metabolism, elimination and toxicological properties. Proteins act as a transport system but concurrently according to "free drug hypothesis" only unbound drug can provide pharmacological effect. Studying of drug-plasma proteins interactions is thus important part of the early stage of drug discovery and development and for this reason is necessary to develop automated high throughput screening assays which lead to higher laboratory efficiency.Capillary electrophoresis seems to be most beneficial approach for this purpose due to its advantages such as low sample consumption, short analysis time, no need for highly purified samples, immobilization or labelling of any of the interacting partners, automation and the possibility of studying both low and high affinity interactions. The disadvantage can be insufficient sensitivity of measuring with UV-VIS detection.In this study capillary electrophoresis with mass spectroscopy detection was used for determination of poorly soluble drug binding parameters like a second-generation sulfonylurea hypoglycemic agent – glimepiride. |
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