Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A

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Authors	KAHOUNOVÁ Zuzana SLABÁKOVÁ Eva BINÓ Lucia REMŠÍK Ján FEDR Radek BOUCHAL Jan KURFŰRSTOVÁ Daniela VRTĚL Radek ŠTUDENT Vladimír JUREČKOVÁ Lucie POROKH Volodymyr HAMPL Aleš SOUČEK Karel
Year of publication	2018
Type	Article in Periodical
Magazine / Source	Stem Cell Research
MU Faculty or unit	Faculty of Medicine
Citation
Web	https://www.sciencedirect.com/science/article/pii/S1873506118302757?via%3Dihub
Doi	http://dx.doi.org/10.1016/j.scr.2018.11.006
Keywords	prostate cancer
Description	A human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5 (TM) Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies.
Related projects:	Centrosomální abnormality u lidských pluripotentních kmenových buněk National research infrastructure for biological and medical imaging Extracelulární faktory ovlivňující metabolismus centrozomů v lidských pluripotentních kmenových buňkách