Předléčebné stanovení radiosenzitivity nádorů hlavy a krku
| Title in English | Prior to treatment radiosensitivity in head and neck cancer |
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| Authors | |
| Year of publication | 2016 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Aims: Head and neck tumour are agressively growing and the oncologic treatment often results in mutilation of the patiens and the quality of life impairment. Thus recently the non-surgical treatment (radiotherapy) is vastly prefered. However, more than 50% of the tumours are not radio-sensitive enought. Unfortunately, still any sensitivizy marker for a useful pre treatment radiosensitivity assesment is missing. Methods: On cancer cell lines the DNA damage is assesed; DNA double strand breaks are visualized by immunofluoroscopy, their count, repair dynamic and capacity in real time) Results: cell lines were separated and cultivated from tissue samples taken from patiens prior to treatment) were in vitro irradiated ( 2Gy, 60 Co) and quantified DNA yH2AX/53PBl foci before, immediatly after irradiation (5min.,) and in the course of the next 24 hours. Results obtained on cancer cells, tumour associated flbroblasts and skin fibroblasts of the same patient were compaired. The preliminary results confirmed higher genomic instability in both tomour cell and tumour associated fibroblast in comparison with normal skinfibroblasts. Conclusion The difference in behaviour between both tumour cell lines and normal flbroblasts in vitro condition after experimental irradiation should be supported by father experimental in vitro and in vivo conditions (clinical tumour responce to radiotherapy and other histopathological and clinical tumour parameters should be futher consederd so that the radiosensitivity could be in vitro estimated prior to treatment decision making. |
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