Tri- and tetravalent mannoclusters cross-link and aggregate BC2L-A lectin from Burkholderia cenocepacia

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Authors	CSÁVÁS Magdolna MALINOVSKÁ Lenka PERRET Florent GYURKÓ Milán ILLYÉS Zita Tünde WIMMEROVÁ Michaela BORBÁS Anikó
Year of publication	2017
Type	Article in Periodical
Magazine / Source	Carbohydrate Research
MU Faculty or unit	Central European Institute of Technology
Citation
Web	https://www.sciencedirect.com/science/article/pii/S0008621516303160?via%3Dihub
Doi	http://dx.doi.org/10.1016/j.carres.2016.11.008
Field	Biochemistry
Keywords	Burkholderia cenocepacia; Mannose-binding lectin; Glycoclusters; Click reaction; Anti-adhesion therapy
Description	The opportunistic Gram-negative bacterium Burkholderia cenocepacia causes lethal infections in cystic fibrosis patients. Multivalent mannoside derivatives were prepared as potential inhibitors of lectin BC2L-A, one of the virulence factors deployed by B. cenocepacia in the infection process. The interaction between the glycoclusters and the mannose binding BC2L-A lectin from B. cenocepacia was examined by isothermal microcalorimetry, surface plasmon resonance, inhibition of yeast agglutination and analytical ultracentrifugation.
Related projects:	Glykoklastry kalix[4]aren/C-oligosacharidy pro studium selektivity interakcí s lektiny Czech Infrastructure for Integrative Structural Biology