Microscopic anatomy of monogenean parasites
| Authors | |
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| Year of publication | 2016 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Using a combined approach of confocal and electron microscopy, adults of monogenean parasite Paradiplozoon homoion were investigated for the body organisation and adaptation to the ectoparasitic lifestyle, with emphasis on worm’s forebody and hindbody. Special attention was given to the parasite’s tegument, digestive, reproductive, and excretory systems. Labelling of filamentous actin with fluorescent phalloidin revealed the musculature of the body wall, buccal suckers, the pharynx as well as some parts of reproductive tract. Staining with chloride carmine and counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. Immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system and some parts of nervous system. |
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