Development of an RP-HPLC method for determination of salicylic acid and its metabolic intermediates from plant biosynthesis pathways
| Authors | |
|---|---|
| Year of publication | 2016 |
| Type | Article in Proceedings |
| Conference | Advances in chromatography and electrophoresis & Chiranal 2016 |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | RP-HPLC; method validation; salicylic acid; gentisic acid; salicylaldehyde; saligenin |
| Description | Salicylic acid (2-hydroxybenzoic acid, SA) belongs to a group of plant growth regulators that have an aromatic ring bearing a hydroxyl group or its functional derivative in the structure. SA functions as an important plant signaling compound that is synthesized in plants as a response to various pathogenic attacks or environmental stress (caused by microbes, insects, herbivores etc.). Two pathways of SA biosynthesis were described in the literature, but the production of SA is still not fully clarified. To better understand the various transformation pathways of SA, analytical methods capable of separating not only SA, but also other relevant compounds from the biotransformation cycles need to be developed. In this study, a reverse-phase high performance liquid chromatography (RP-HPLC) method for determination of SA and metabolic intermediates derived from its biosynthetic pathways (salicin (2-(hydroxymethyl)phenyl-beta-D-glucopyranoside), helicin (salicylaldehyde beta-D-glucoside), gentisic acid (2,5-dihydroxybenzoic acid), saligenin (2-hydroxybenzyl alcohol) and salicylaldehyde (2-hydroxybenzaldehyde) was developed. The method was then applied for analysis of samples from white willow (Salix alba), as willow cortex is well-known as a natural source of salicin and salicylic acid. |
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