Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions

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Authors	ZOVČÁKOVÁ Monika ŠPANOVÁ Alena PANTŮČEK Roman DOŠKAŘ Jiří RITTICH Bohuslav
Year of publication	2016
Type	Article in Periodical
Magazine / Source	Analytical Biochemistry
MU Faculty or unit	Faculty of Science
Citation
Web	http://www.sciencedirect.com/science/article/pii/S0003269716300884
Doi	http://dx.doi.org/10.1016/j.ab.2016.05.013
Field	Genetics and molecular biology
Keywords	Lanthanide Nd3+ ions; Plasmid DNA; Plasmid size; Pulsed-field agarose gel electrophoresis; Staphylococcus aureus
Attached files	1-s2.0-S0003269716300884-main.pdf
Description	Staphylococcus aureus plasmids are the main factor in the spreading of antibacterial resistance among bacterial strains that has emerged on a worldwide scale. Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd3+ ions (non-enzymatic cleavage agent) in Hepes buffer (pH 7.5) at 70 °C. Topological forms of plasmids—closed circular (ccc), open circular (oc), and linear (lin)—produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.
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