Reward related neurotransmitter changes in a model of depression: An in vivo microdialysis study

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Authors	RUDÁ Jana AMCHOVÁ Petra HAVLICKOVA Tereza JERABEK Pavel BABINSKÁ Zuzana KACER Petr SYSLOVA Kamila ŠULCOVÁ Alexandra SUSTKOVA-FISEROVA Magdalena
Year of publication	2015
Type	Article in Periodical
Magazine / Source	The World Journal of Biological Psychiatry
MU Faculty or unit	Faculty of Medicine
Citation
Web	http://www.tandfonline.com/doi/full/10.3109/15622975.2015.1077991#abstract
Doi	http://dx.doi.org/10.3109/15622975.2015.1077991
Field	Pharmacology and pharmaceutical chemistry
Keywords	Depression; in vivo microdialysis; methamphetamine; olfactory bulbectomy; rats
Attached files	EL_1313657.pdf
Description	OBJECTIVES: The self-medication hypothesis assumes that symptoms related to potential monoaminergic deficits in depression may be relieved by drug abuse. The aim of this study was to elucidate the neurotransmitter changes in a rat model of depression by measuring their levels in the nucleus accumbens shell, which is typically involved in the drug of abuse acquisition mechanism. METHODS: Depression was modelled by the olfactory bulbectomy (OBX) in Wistar male rats. In vivo microdialysis was performed, starting from the baseline and following after a single methamphetamine injection and behaviour was monitored. The determination of neurotransmitters and their metabolites was performed by high-performance liquid chromatography combined with mass spectrometry. RESULTS: OBX animals had lower basal levels of dopamine and serotonin and their metabolites. However, GABA and glutamate levels were increased. The methamphetamine injection induced stronger dopamine and serotonin release in the OBX rats and lower release of glutamate in comparison with sham-operated rats; GABA levels did not differ significantly. CONCLUSIONS: This study provides an evidence of mesolimbic neurotransmitter changes in the rat model of depression which may elucidate mechanisms underlying intravenous self-administration studies in which OBX rats were demonstrated to have higher drug intake in comparison to intact controls.
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