Identification of individual biofilm-forming bacterial cells using Raman tweezers

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Authors

SAMEK Ota BERNATOVÁ Silvie JEŽEK Jan ŠILER Martin ŠERÝ Mojmir KRZYŽÁNEK Vladislav HRUBANOVÁ Kamila ZEMÁNEK Pavel HOLÁ Veronika RŮŽIČKA Filip

Year of publication 2015
Type Article in Periodical
Magazine / Source Journal of Biomedical Optics
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1117/1.JBO.20.5.051038
Field Microbiology, virology
Keywords Raman tweezers; Staphylococcus epidermidis; biofilm
Description A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral "Raman fingerprints" obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilmnegative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.
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