Characterisation of an unusual telomere motif (TTTTTTAGGG)(n) in the plant Cestrum elegans (Solanaceae), a species with a large genome
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Periodical |
| Magazine / Source | The Plant Journal |
| MU Faculty or unit | |
| Citation | |
| Web | http://onlinelibrary.wiley.com/doi/10.1111/tpj.12839/epdf |
| Doi | http://dx.doi.org/10.1111/tpj.12839 |
| Field | Genetics and molecular biology |
| Keywords | Cestrum elegans; NGS analysis; RepeatExplorer; telomerase; telomeric sequence; unusual telomere; GenBank KM573817-573822 |
| Attached files | |
| Description | The characterization of unusual telomere sequence sheds light on patterns of telomere evolution, maintenance and function. Plant species from the closely related genera Cestrum, Vestia and Sessea (family Solanaceae) lack known plant telomeric sequences. Here we characterize the telomere of Cestrum elegans, work that was a challenge because of its large genome size and few chromosomes (1C 9.76pg; n=8). Significance Statement We previously found that plants from related genera Cestrum, Vestia and Sessea (Solanaceae) lack known telomeric sequences. Here we characterise the Cestrum elegans telomere and the mechanism of its synthesis. We developed an approach combining NGS of untreated and terminally-truncated genomic DNA. We identified, using the RepeatExplorer bioinformatic pipeline, an unusual telomere repeat (TTTTTTAGGG)(n) synthesized by telomerase. That finding will enable studies on evolutionary divergence of telomeres and telomerase, shedding light on telomere interactions and functions. |
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