A Novel Electrochemical Sensor for the Detection of Purine Derivatives
| Authors | |
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| Year of publication | 2013 |
| Type | Article in Proceedings |
| Conference | 64th Annual Meeting of the International Society of Electrochemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Physical chemistry and theoretical chemistry |
| Keywords | electrochemical sensor; purine derivatives; monovalent copper; complex Cu(I)-purine; EVLS; |
| Description | A simple and cheap electrochemical sensor for the sensitive detection of purine derivatives was designed. The purine sensor is based on a graphite electrode modified with cuprous ions. Monovalent copper ions generated in situ on the electrode surface are able to form, with purine derivatives, sparingly soluble complexes strongly adsorbed on the electrode surface. These complexes provide a sensitive qualitative and quantitative monitoring of individual purines and their mixtures not only by means of oxidation signals of Cu(I)-purine complexes but also by means of oxidation signals of the corresponding purine. Signal amplification of the electrochemical sensor consists of both an increase of surface concentration of the purine derivative through adsorption with a low soluble Cu(I)-purine complex and the second signal processing using EVLS – Elimination voltammetry with Linear Scan. The EVLS is an unconventional electrochemical method capable of eliminating selected particular currents (diffusion, charging, kinetic current, etc.) from the total voltammetric current. The elimination procedure is very fast and simple and requires only three total voltammetric curves recorded at three different scan rates. For the sensing, the application of EVLS function E4 (conserving the diffusion current component) has a significant advantage because in the case of adsorbed electroactive species it provides a special peak-counterpeak signal. The invented sensor relates generally to diagnostic systems and methods of improved electrochemical measurement of purines in food, beverage and body fluid samples. Moreover, the simultaneous electrochemical monitoring of metabolites related to the purine catabolism pathway is promising. |
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