The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. II. Selection of Direct Kjeldahl Analysis and Its Preliminary Performance Parameters

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Authors

VINKLÁRKOVÁ Bára CHROMÝ Vratislav ŠPRONGL Luděk BITTOVÁ Miroslava RIKANOVÁ Milena OHNÚTKOVÁ Ivana ŽALUDOVÁ Lenka

Year of publication 2015
Type Article in Periodical
Magazine / Source Critical Reviews in Analytical Chemistry
MU Faculty or unit

Faculty of Science

Citation
Web http://www.tandfonline.com/doi/full/10.1080/10408347.2014.892821#abstract
Doi http://dx.doi.org/10.1080/10408347.2014.892821
Field Analytic chemistry
Keywords serum-based reference materials; Kjeldahl method; total protein
Description To select a Kjeldahl procedure suitable for the determination of total protein in reference materials used in laboratory medicine, we reviewed in our previous article Kjeldahl methods adopted by clinical chemistry and found an indirect two-step analysis by total Kjeldahl nitrogen corrected for its nonprotein nitrogen and a direct analysis made on isolated protein precipitates. In this article, we compare both procedures on various reference materials. An indirect Kjeldahl method gave falsely lower results than a direct analysis. Preliminary performance parameters qualify the direct Kjeldahl analysis as a suitable primary reference procedure for the certification of total protein in reference laboratories.
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