The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. I. A Review of Kjeldahl Methods Adopted by Laboratory Medicine

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Authors

CHROMÝ Vratislav VINKLÁRKOVÁ Bára ŠPRONGL Luděk BITTOVÁ Miroslava

Year of publication 2015
Type Article in Periodical
Magazine / Source Critical Reviews in Analytical Chemistry
MU Faculty or unit

Faculty of Science

Citation
Web http://www.tandfonline.com/doi/abs/10.1080/10408347.2014.892820#.VPRShSwkUXk
Doi http://dx.doi.org/10.1080/10408347.2014.892820
Field Analytic chemistry
Keywords calibration; reference materials; Kjeldahl methods; clinical chemistry; total protein
Description We found previously that albumin-calibrated total protein in certified reference materials causes unacceptable positive bias in analysis of human sera. The simplest way to cure this defect is the use of human-based serum/plasma standards calibrated by the Kjeldahl method. Such standards, commutative with serum samples, will compensate for bias caused by lipids and bilirubin in most human sera. To find a suitable primary reference procedure for total protein in reference materials, we reviewed Kjeldahl methods adopted by laboratory medicine. We found two methods recommended for total protein in human samples: an indirect analysis based on total Kjeldahl nitrogen corrected for its nonprotein nitrogen and a direct analysis made on isolated protein precipitates. The methods found will be assessed in a subsequent article.
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