Cystatins of the parasitic Nematodes - Trichinella spiralis, T. britovi and T. pseudospiralis
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| Year of publication | 2014 |
| Type | Appeared in Conference without Proceedings |
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| Description | The nematodes of the genus Trichinella are intracellular parasites which invade the cells of small intestine and skeletal muscles. They infect a broad range of worldwide distributed hosts, like mammals, birds and reptiles. All Trichinella spp. are zoonotic; six of twelve genotypes have been detected in humans. The serious human disease is called trichinellosis and it has been documented in 55 countries. Our work is focused on cystatins - a reversible inhibitors of cystein peptidases of three trichinella species - Trichinella spiralis, T. britovi and T. pseudospiralis. Cystatins are compounds of excretory-secretory products (ESP) which are released by muscle larvae (L1) into the external environment. They are involved in many essential biological processes like growth, development, digestion and migration and also in manipulation of the host immunne system by parasite. Up to now, the specific primers were designed for “multi cystatin-like domain” gene of T. spiralis, T. britovi and T. pseudospiralis; this gene was amplified, cloned and sequenced. The comparison of sequential data showed that cystatin genes of T. spiralis and T. britovi are significantly homologous but differs to gene conserved motifs of T. pseudospiralis cystatin and in number of domains. The recombinant forms of cystatins of mentioned trichinella species will be prepared and their biochemical properties will be compared. |
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