Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis

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Authors

KUBÁŇ Petr GREGUŠ Michal POKOJOVÁ Eva SKŘIČKOVÁ Jana FORET František

Year of publication 2014
Type Article in Periodical
Magazine / Source Journal of Chromatography A
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.sciencedirect.com/science/article/pii/S002196731401036X
Doi http://dx.doi.org/10.1016/j.chroma.2014.06.091
Field Analytic chemistry
Keywords Capillary electrophoresis; Contactless conductivity detection; Ions; Cystic fibrosis; Principal component analysis
Attached files
Description A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1 mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3 min in a background electrolyte containing 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine and 2 mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed. (C) 2014 Elsevier B.V. All rights reserved.
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