HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes

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Authors

MIDLIK Adam ŠEBRLOVÁ Kristýna KUŠNYEROVÁ Ivana DOVRTĚLOVÁ Gabriela NOSKOVÁ Kristýna JUŘICA Jan TÁBORSKÁ Eva PEŠ Ondřej

Year of publication 2014
Type Article in Proceedings
Conference Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 & 15th Czech - Slovak Spectroscopic Conference
MU Faculty or unit

Faculty of Medicine

Citation
Field Biochemistry
Keywords LC MS; benzophenanthridine alkaloids; metabolites; microsomes
Description Quaternary benzophenanthridine alkaloids (QBAs) are a group of isoquinoline derivatives produced by plants of the Papaveraceae, Fumariaceae and Rutaceae family. Presently they are studied intensively because of their biological activity. Anti-inflammatory, anti-microbial, anti-fungal, antiparasitic, as well as pro-apoptotic and anti-tumor effects of QBAs have been reported. Determination of toxicity of these potential drugs is based on metabolic studies of major, commercially available sanguinarine (SA) and chelerythrine (CHE). However, metabolic fate and pharmacological activity of minor QBAs, such as chelilutine (CL), chelirubine (CR), sanguilutine (SL), sanguirubine (SR) and macarpine (MA), might differ considerably, despite their structural similarity. Rat liver microsomes (RLMs), subcellular particles containing metabolizing enzymes, such as a cytochrome P450 family, provide a simple metabolic model for in vitro investigation before carrying out in vivo tests.
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