Monitoring the influence of antibiotic exposure using Raman spectroscopy

• Authors: SAMEK Ota; ZEMÁNEK Pavel; BERNATOVÁ Silvie; JEZEK Jan; SERY Mojmir; JAKL Petr; ŠILER Martin; RŮŽIČKA Filip
• Year of publication: 2014
• Type: Article in Proceedings
• Conference: Proceedings of SPIE : BIOMEDICAL VIBRATIONAL SPECTROSCOPY VI: ADVANCES IN RESEARCH AND INDUSTRY
• MU Faculty or unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1117/12.2038595
• Field: Medical equipment
• Keywords: Raman spectroscopy; bacteria; antibiotics; bacteriostatic action; bactericidal action
• Description: Here we report on combination of the data obtained from MICs (minimum inhibitory concentrations) with information of microoragnisms fingerprint provided by Raman spectroscopy. In our feasibility study we could follow mechanisms of the bacteriostatic versus bactericidal action on biofilm-positive Staphylococcus epidermidis simply by monitoring Raman bands corresponding to DNA translating the changes introduced by selected antibiotics. The Raman spectra of Staphylococcus epidermidis treated with a bacteriostatic agent show little effect on DNA which is in contrast with the action of a bactericidal agent where decreased in dedicated Raman spectra signal strength suggests DNA fragmentation. Moreover, we demonstrate that Raman tweezers are indeed able to distinguish strains of biofilm-forming (biofilm-positive) and biofilm-negative Staphylococcus epidermidis strains using principal component analysis (PCA).

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