Electrochemical and Spectral Analysis of Cytosine and Guanine Homo-oligodeoxynucleotides
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | XXXIV. Moderní Elektrochemické Metody |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.bestservis.eu/upload/file/Sbornik_metody14.pdf |
| Field | Physical chemistry and theoretical chemistry |
| Keywords | Oligodeoxynucleotides (ODNs); Homo-ODN; Cytosin (C); Guanin (G); Cyclic Voltammetry (CV); Linear Sweep Voltammetry (LSV); Elimination Voltammetric procedure (EVP); Circular Dichroism (CD) |
| Description | Oligodeoxynucleotides (ODNs) with repetitive cytosine (C) or guanine (G) sequences are observed in the promoter region of the oncogenes and of human telomeric DNA. Here we present electrochemical and spectral studies of short C or G homo-ODNs in solutions with different pH. Electrochemistry was represented by linear sweep or cyclic voltammetry in connection with a hanging mercury drop electrode (HMDE). As a spectral method circular dichroism was used. It was found that cytosine ODNs form stable structures, the so-called i-motifs and guanine ODN structures form tetraplex via a G-quartet. Both structures are dependent on pH and the ODN chain length. |
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