Study of interaction of benzo[c]phenanthridine alkaloids with DNA

Authors

PAROULEK Petr RÁJECKÝ Michal PĚNČÍKOVÁ Kristýna TÁBORSKÝ Petr

Year of publication 2014
Type Conference abstract
Citation PAROULEK, Petr, Michal RÁJECKÝ, Kristýna PĚNČÍKOVÁ and Petr TÁBORSKÝ. Study of interaction of benzo[c]phenanthridine alkaloids with DNA. In European symposium on atomic spectrometry ESAS 2014 & 15th Czech-Slovak spectroscopic conference. 2014. ISBN 978-80-905704-1-2.
Description G-quadruplex (GQ) is one of non-canonical structures of nucleic acids (NA). Structures of intramolecular GQs (formed by only one strand of NA) can be distinguished into two groups according to sequence orientation: parallel and anti-parallel GQs [1]. The effect of selected benzo[c]phenanthridine and protoberberine alkaloids from plants of Papaveraceae family [2] on stability of parallel (sequence from c- kit oncogene promoter) and anti-parallel (human telomeric sequence) GQ was studied. Stabilization (in terms of melting point shifts) of GQs by interaction with macarpine, sanguilutine, sanguinarubine, sanguinarine, chelerythrine and corysamine was followed by UV-vis and CD spectrometry. Results showed that all tested alkaloids except macarpine have a stabilizing effect on human telomeric sequence (?T m = 3-17 °C). Parallel sequence from c-kit oncogene promoter was stabilized by all alkaloids.
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