Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

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Authors	BEDNAŘÍK Antonín KUBA Pavel MOSKOVETS Eugene TOMALOVÁ Iva KRÁSENSKÝ Pavel HOUŠKA Pavel PREISLER Jan
Year of publication	2014
Type	Article in Periodical
Magazine / Source	Analytical Chemistry
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://pubs.acs.org/doi/abs/10.1021/ac402823n
Doi	http://dx.doi.org/10.1021/ac402823n
Field	Analytic chemistry
Keywords	Mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput
Description	This work shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in 11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
Related projects:	Analýza biomolekul hmotnostní spektrometrií s laserovou desorpcí/ionizací za účasti nanomateriálů CEITEC - Central European Institute of Technology Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci