Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

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Authors

BEDNAŘÍK Antonín KUBA Pavel MOSKOVETS Eugene TOMALOVÁ Iva KRÁSENSKÝ Pavel HOUŠKA Pavel PREISLER Jan

Year of publication 2014
Type Article in Periodical
Magazine / Source Analytical Chemistry
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://pubs.acs.org/doi/abs/10.1021/ac402823n
Doi http://dx.doi.org/10.1021/ac402823n
Field Analytic chemistry
Keywords Mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput
Description This work shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in 11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
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