The More the Merrier: Recent Hybridization and Polyploidy in Cardamine

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.

Authors	MANDÁKOVÁ Terezie KOVAŘÍK Aleš ZOZOMOVÁ-LIHOVÁ Judita SHIMIZU-INATSUGI Rie SHIMIZU Kentaro K. MUMMENHOFF Klaus MARHOLD Karol LYSÁK Martin
Year of publication	2013
Type	Article in Periodical
Magazine / Source	Plant Cell
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://www.plantcell.org/content/early/2013/09/30/tpc.113.114405.short?rss=1
Doi	http://dx.doi.org/10.1105/tpc.113.114405
Field	Genetics and molecular biology
Keywords	CHROMOSOME-NUMBER REDUCTION; MIXED-PLOIDY POPULATIONS; NUCLEAR RIBOSOMAL DNA; COMPARATIVE GENOMICS; ARABIDOPSIS-THALIANA; GENETIC-VARIATION; VASCULAR PLANTS; FLOW-CYTOMETRY; BRASSICACEAE; EVOLUTION
Attached files	Mandakova_Cardamine_Urnerboden_story_TPC2013.pdf
Description	This article describes the use of cytogenomic and molecular approaches to explore the origin and evolution of Cardamine schulzii, a textbook example of a recent allopolyploid, in its similar to 110-year history of human-induced hybridization and allopolyploidy in the Swiss Alps. Triploids are typically viewed as bridges between diploids and tetraploids but rarely as parental genomes of high-level hybrids and polyploids. The genome of the triploid semifertile hybrid Cardamine 3 insueta (2n = 24, RRA) was shown to combine the parental genomes of two diploid (2n = 2x = 16) species, Cardamine amara (AA) and Cardamine rivularis (RR). These parental genomes have remained structurally stable within the triploid genome over the > 100 years since its origin. Furthermore, we provide compelling evidence that the alleged recent polyploid C. schulzii is not an autohexaploid derivative of C. x insueta. Instead, at least two hybridization events involving C. x insueta and the hypotetraploid Cardamine pratensis (PPPP, 2n = 4x-2 = 30) have resulted in the origin of the trigenomic hypopentaploid (2n = 5x22 = 38, PPRRA) and hypohexaploid (2n = 6x-2 = 46, PPPPRA). These data show that the semifertile triploid hybrid can promote a merger of three different genomes and demonstrate how important it is to reexamine the routinely repeated textbook examples using modern techniques.
Related projects:	Evoluce genomu alopolyploidních řeřišnic (Cardamine) rozdílného fylogenetického stáří CEITEC - Central European Institute of Technology EVOGEN - Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace