Immobilized Enzyme Reactor for On-Line Studies of Drug Metabolism Mediated by Cytochrome P450 2C9 Isoform

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Authors	SCHEJBAL Jan ŘEMÍNEK Roman ZEMAN Lukáš ZEISBERGEROVÁ Marta GLATZ Zdeněk
Year of publication	2013
Type	Article in Periodical
Magazine / Source	Chemické listy
MU Faculty or unit	Faculty of Science
Citation
Web	http://www.chemicke-listy.cz/docs/full/2013_s3_s420-s453.pdf
Field	Biochemistry
Keywords	Capillary Electrophoresis; Drug Metabolism; CYP2C9; Diclofenac; Immobilization; IMER
Description	Drug biotransformation mediated by cytochrome P450 enzymes (CYP) is a pivotal factor in the early developmental stages of new drugs. Metabolism appraisal of every hit thus constitutes the inseparable part of ADME/tox screenings. Enzyme immobilization represents a useful approach in this field because of CYP recycling and simplification of sequential analyses by providing protein-free samples. A new method for immobilization of therapeutically important cytochrome P450 2C9 isoform (CYP2C9) on magnetic microparticles SiMAG Carboxyl is thus presented.
Related projects:	Miniaturized on-line drug metabolism system based on capillary electrophoresis BBC - Vytvoření multidisciplinárního výzkumného a vzdělávacího centra bioanalytických technologií