Naphthalene-2,3-dicarbaldehyde derivatization of amino acids - an improved technique for minimization of benzoin condensation

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Authors

DĚDOVÁ Tereza CELÁ Andrea MÁDR Aleš GLATZ Zdeněk

Year of publication 2013
Type Article in Periodical
Magazine / Source Chemické listy
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords capillary electrophoresis; LIF; Amino acids;
Description Amino acids (AA) have no native fluorescence thus chemical modification (so called derivatization) of AA has to be performed prior the detection by a fluorescent detector. There is a lot of derivatization reagents commercially available differing in reactivity, excitation and emission wavelengths, quantum yield, product stability etc. Naphtalene-2,3-dicarbaldehyde (NDA) is a non-fluorescent reagent giving fluorescent product of primary amines. Derivatization procedure is quite simple, however has to be optimized to minimize undesired by-products of benzoin condensation. The by-products can exhibit fluorescence or form crystals which can clog capillary. The novel derivatization protocol using NDA for AA derivatization is subject of this contribution.
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