Study of the most common additives affecting electro-osmotic flow for use in capillary electrophoresis with contactless conductivity detection setup
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | Chemické listy |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | capillary electrophoresis; electroosmotic flow; contactless conductivity detection; |
| Description | Electrophoretic and electroosmotic phenomena occur in capillary electrophoresis. Both phenomena manifest in dependence on in-capillary conditions. A lot of additives affecting electro-osmotic flow dynamically have been introduced over the years. However no comprehensive study has been published about effects of these additives on the signal of the contactless conductivity detection (C4D) yet. C4D is nonspecific detection technique suitable for a direct analysis of ionic species, but it is sensitive to a composition of background electrolyte as well. The subject of this contribution was present the most common additives used for electro-osmotic flow alteration, discus their suitability considering both magnitude of electro-osmotic flow affection and the effect on the signal of the C4D. |
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