Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels

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Authors	KONEČNÁ Hana FRIDRICHOVÁ Danuše LOCHMANOVÁ Gabriela BÁRTA Jan ZDRÁHAL Zbyněk
Year of publication	2013
Type	Article in Periodical
Magazine / Source	Electrophoresis
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	http://dx.doi.org/10.1002/elps.201300084
Field	Analytic chemistry
Keywords	2DE; Mixed detergent micelles; Tris-Tricine gel
Description	Application of Tris-N-[Tris(hydroxymethyl)methyl]glycine gels for 2DE is hampered by formation of mixed CHAPS-SDS micelles resulting in typical swirling pattern in the low mass range, which makes reliable quantitative and qualitative gel evaluation impossible. Modification of 2DE strip equilibration procedure prevented the direct interaction between both detergents during equilibration process, thus substantially improving gel separation.
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