Protein engineering of beta-D-mannosidase

Demo,  Gabriel; Fliedrova, Barbora; Weignerova, Lenka; Wimmerová,  Michaela

Protein engineering of beta-D-mannosidase

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Authors	DEMO Gabriel FLIEDROVA Barbora WEIGNEROVA Lenka WIMMEROVÁ Michaela
Year of publication	2013
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Description	N-Acetyl-D-mannosamine (ManNAc) is often used as a precursor in the chemical or enzymatic synthesis of the neuraminic acids found in glycolipids and glycoproteins. ManNAc is also a synthetic intermediate for a number of carbohydrate-derived families of biologically active compounds and pharmaceutical candidates. For this reason ManNAc is of keen interest to scientists around the world. The main goal of this work, because no N-Acetyl-mannosaminidase has been identified in the nature, is to produce N-acetyl-mannosaminidase via mutagenesis of beta-D-mannosidase and to apply this new enzyme in possible enzymatic synthesis of structures containing ManNAc.
Related projects:	Enzymy metabolismu mannosidů v přípravě N-acetyl-mannosaminových struktur