Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger

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Authors	DEMO Gabriel FLIEDROVA Barbora WEIGNEROVA Lenka WIMMEROVÁ Michaela
Year of publication	2013
Type	Article in Periodical
Magazine / Source	Acta crystallographica. Section F
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	http://dx.doi.org/10.1107/S1744309113002522
Field	Biochemistry
Keywords	Mannosidase; Aspergillus niger; Expression; Crystallization
Description	beta-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal beta-linked mannoside in various oligomeric saccharide structures. beta-Mannosidase from Asperigillus niger was expressed in Pichia pastoris and purified to clear homogeneity. beta-mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 A resolution. The crystal belonged to space group P1. Monomeric structure was observed in the asymmetric unit.
Related projects:	Enzymy metabolismu mannosidů v přípravě N-acetyl-mannosaminových struktur CEITEC - Central European Institute of Technology