Circular Dichroism Spectroscopy of DNA: From Duplexes to Quadruplexes

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Authors	VORLÍČKOVÁ Michaela KEJNOVSKÁ Iva BEDNÁŘOVÁ Klára RENČIUK Daniel KYPR Jaroslav
Year of publication	2012
Type	Article in Periodical
Magazine / Source	Chirality
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://www.ncbi.nlm.nih.gov/pubmed/22696273
Doi	http://dx.doi.org/10.1002/chir.22064
Field	Biophysics
Keywords	circular dichroism; nucleic acid conformations; structural transitions; B form; A form; Z form; trinucleotide repeats; RNA
Attached files	Vorlic_chir_22064_Rev_EV.pdf
Description	Nucleic acids bear the genetic information and participate in its expression and evolution during replication, repair, recombination, transcription, and translation. These phenomena are mostly based on recognition of nucleic acids by proteins. The major factor enabling the specific recognition is structure. Circular dichroism (CD) spectroscopy is very useful to study secondary structures of nucleic acids, in general, and DNA, in particular. CD sensitively reflects isomerizations among distinct conformational states. The isomerizations may operate as molecular switches regulating various physiological or pathological processes. Here, we review CD spectra of nucleic acids, beginning with early studies on natural DNA molecules through analyses of synthetic polynucleotides to study of selected genomic fragments. Chirality 24:691698, 2012. (c) 2012 Wiley Periodicals, Inc.
Related projects:	Biophysics and bioinformatics of genomic DNA fragments very rich in guanine and adenine CEITEC - Central European Institute of Technology