Project information
Identification of molecular components and the mechanism of polar targeting of PIN auxin transport proteins in Arabidopsis thaliana
- Project Identification
- IAA601630703
- Project Period
- 1/2007 - 12/2011
- Investor / Pogramme / Project type
-
Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
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Central European Institute of Technology
- Mgr. Jiří Friml, Dr. rer. nat.
- doc. RNDr. Jan Hejátko, Ph.D.
- Mgr. Eva Paděrová
- Iveta Strouhalová
- Ing. Radka Zemová, Ph.D.
- Keywords
- auxin transport, polar targeting, PIN proteins, forward genetics, map-based cloning, Arabidopsis thaliana
The directional transport of the plant signalling molecule auxin is a unique process mediating a wide variety of developmental roles including organogenesis, patterning and tropisms. Direction of intercellular auxin flow is determined by a dynamic polar localisation of auxin efflux catalysts - Arabidopsis thaliana PIN proteins. Hence decision about PIN polar targeting is crucial for mediating diverse developmental responses. We intend to elucidate mechanism of polar targeting in plants by identifying molecular components of PIN polar localisation by molecular genetic approaches. Two mutant screens will be performed: (i) screen for suppressors of 35S::PIN1 dominant overexpression phenotype and (ii) for mutants with defected polar exocytosis of PIN1:GFP protein. Map-based cloning of these mutants will reveal additional molecular components of asymmetric auxin distribution and novel components of cell polarity and protein targeting in plants.
Publications
Total number of publications: 13
2009
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ER-localized PIN5 auxin transporter mediates subcellular homeostasis of phytohormone auxin
Nature, year: 2009, volume: Neuveden, edition: Neuveden
2008
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Alternative telomere lengthening in development of normal and telomerase knock-out plants
Year: 2008, type: Conference abstract
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Role of alternative telomere lengthening unmasked in telomerase knock-out mutant plants
Plant Molecular Biology, year: 2008, volume: 60, edition: February