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Project information
Nové flavin-dependentní enzymy Paracoccus denitrificans: reakční mechanismy, metabolické funkce a úloha v buněčném oxidačním stresu

Information

This project doesn't include Faculty of Economics and Administration. It includes Faculty of Science. Official project website can be found on muni.cz.
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Project Identification
GAP503/12/0369
Project Period
1/2012 - 12/2015
Investor / Pogramme / Project type
Czech Science Foundation
  • Standard Projects
MU Faculty or unit
Faculty of Science

Projekt se zabyva dvema enzymy,
avin reduktasou FerA a chinonreduktasou FerB, spojenymi s genero-
vanm nebo zvladanm oxidacnho stresu u bakterie Paracoccus denitri cans, casto nazyvane
"
volne zijc
mitochondrie\. Bude izolovan gen pro FerA a pripraveny odpovdajc rekombinantn protein bude charak-
terizovan enzymologicky, krystalogra cky a pomoc mstne rzene mutageneze. Bude stanoveno, zda FerA
pusob samostatne nebo v kombinaci s dalsmi proteiny. V pokusech in vitro bude kvanti kovana produkce
superoxidu enzymem FerB a testovana hypoteza, ze FerB je schopen redukovat membranovy ubichinon. U divokeho kmene a mutantnch kmenu bez FerA resp. FerB nebo s jejich zvysenou produkc bude porov-
nan stupen peroxidace membranovych lipidu. Proteomicka analyza bunek vystavenych redoxnm stresorum
pomuze pri identi kaci clenu redoxnho regulonu.

Publications

Total number of publications: 6

2017

2016

2015

2014

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