Project information
The use of combination of laser microbeam and cytometric techniques in the study of the structure and dynamics of human genome
- Project Identification
- IAA1065203
- Project Period
- 1/2002 - 12/2006
- Investor / Pogramme / Project type
-
Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- optical-tweezers; optical-scalpel; high-resolution-cytometer; confocal- microscopy; chromatin-struct
- Cooperating Organization
-
Institute of Biophysics
- Responsible person Ing. Emilie Lukášová, CSc.
- Responsible person prof. RNDr. Pavel Zemánek, Ph.D.
- Responsible person prof. RNDr. Miroslav Liška, DrSc.
The aim of the project is to use the methods and experimental systems of optical tweezers, optical scalpel, and automated cytometer, that were developed using the finantial support of the previous scientific projects, for the study of the mechanical properties and arrangement of the interphase chromatin in the nuclei of living and fixed human cells. Theoretical and experimental experiences of the interdisciplinary team of applicants will be used for: - fluorescence labelling of chromatin in living cells, - laser induced fusion of labelled cells, - topologic analyses of the dye distribution inside living and fixed cells, - insertion of dyed nanoparticle into the living cell nucleus, optical confinement of this probe and detection of the region which is not accesible for the probe, - study of the mechanical properties of the chromatin using optical trapping of suitable probes. The knowledge obtained by these techniques will be used for development of new diagnostic methods.
Results
Publications
Total number of publications: 12
2007
-
Chromatin dynamics during DSB repair
Biochimica et Biophysica Acta, Molecular Cell Research, year: 2007, volume: 1773, edition: 10
2006
-
Analýza lokalizace a interakce fluorescenčně značených molekul v živých i fixovaných lidských buňkách.
Year: 2006, type: Conference abstract
-
Directional motion of foreign plasmid DNA to nuclear HP1 foci
Chromosome Research, year: 2006, volume: 14, edition: 5
-
Optical tracking of microobjects within living cells
Proceedings of SPIE, year: 2006
2004
-
Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells
Biophysics of the Genome, year: 2004
-
Dynamics of HP1 transgene loci movement and silencing in living cells
Biophysics of the Genome, year: 2004
-
In vivo imaging of cell interior using automated high-resolution cytometry
Biophysics of the Genome, year: 2004
-
Methylation of histone H3K9 in human blood cells and in granulocytes of patients with myeloid leukemia
Biophysics of the Genome, year: 2004
-
Topography of genetic loci in the nuclei of cells of colorectal carcinoma and adjacent tissue of colonic epithelium
Chromosoma, year: 2004, volume: 112, edition: 5
2003
-
Detailed studies of the human genome 3D structure
Biophysics of the Genome, year: 2003